Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


Real-Time Analysis of Mouse Genotypes

Real-Time Analysis of Mouse Genotypes - Real-Time PCR and Quantitative PCR Forum

Real-Time Analysis of Mouse Genotypes - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-20-2007, 03:12 AM
oBWhat's Avatar
Graduate Student
Points: 3,826, Level: 41 Points: 3,826, Level: 41 Points: 3,826, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 124
Thanks: 0
Thanked 7 Times in 7 Posts
Exclamation Real-Time Analysis of Mouse Genotypes



Hey all,
I have a question about Real-Time Analysis of Mouse Genotypes. I need to analyze the genotypes of mice that are homozygous and heterozygous. How can I use real-time to assess genotype of the transgenic mice?

Does expression always correspond to dosage?

Southern blot analysis seems a better approach to me!... tell me please if I may be wrong.

Thanks!

Ob
Reply With Quote
  #2  
Old 05-18-2007, 06:32 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Real-Time Analysis of Mouse Genotypes

You need to know the exact location of the SNP in your sequence. Then design an assay with primers that identify SNP bases. You'll need 2 pairs. One for wild tytpe and the other one for the mutant.
Reply With Quote
  #3  
Old 05-18-2007, 06:32 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Real-Time Analysis of Mouse Genotypes

Southern blot is not very accurate and may give false positives
Reply With Quote
  #4  
Old 05-18-2007, 06:35 AM
Pipette Filler
Points: 1,618, Level: 24 Points: 1,618, Level: 24 Points: 1,618, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 14
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Real-Time Analysis of Mouse Genotypes

If you need a software that can design SNP genotyping assays, get Beacon Designer or AlleleID. They will help you do [Only registered users see links. ] and design of molecular probes such as TaqMan probes or molecular beacons. These primers can be used for your SNP genotyping and detection

Last edited by admin; 04-26-2008 at 08:18 AM.
Reply With Quote
Reply

Tags
analysis , genotypes , mouse , realtime


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
the absolutely final complete collection of ideas blochee Physics Forum 0 06-14-2007 10:30 PM
The Achilles Heel of String Theory. S D Rodrian Physics Forum 7 07-08-2006 02:40 PM
Moving Dimensions Theory Explains Time in Both QM & SR: Roger Penrose Has Been Seeking Moving Dimensions Theory jollyrogership@yahoo.com Physics Forum 10 07-17-2005 12:33 PM


All times are GMT. The time now is 10:14 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.17887 seconds with 16 queries