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02-20-2007, 02:12 AM
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 Real-Time Analysis of Mouse Genotypes Hey all, I have a question about Real-Time Analysis of Mouse Genotypes. I need to analyze the genotypes of mice that are homozygous and heterozygous. How can I use real-time to assess genotype of the transgenic mice? Does expression always correspond to dosage? Southern blot analysis seems a better approach to me!... tell me please if I may be wrong. Thanks! Ob        |
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05-18-2007, 06:32 AM
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 Re: Real-Time Analysis of Mouse Genotypes You need to know the exact location of the SNP in your sequence. Then design an assay with primers that identify SNP bases. You'll need 2 pairs. One for wild tytpe and the other one for the mutant. |
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05-18-2007, 06:32 AM
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| Re: Real-Time Analysis of Mouse Genotypes Southern blot is not very accurate and may give false positives |
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05-18-2007, 06:35 AM
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| Re: Real-Time Analysis of Mouse Genotypes If you need a software that can design SNP genotyping assays, get Beacon Designer or AlleleID. They will help you do real time PCR primer design and design of molecular probes such as TaqMan probes or molecular beacons. These primers can be used for your SNP genotyping and detection Last edited by admin; 04-26-2008 at 08:18 AM. |
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