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Real-Time Analysis of Mouse Genotypes

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Old 02-20-2007, 02:12 AM
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Exclamation Real-Time Analysis of Mouse Genotypes

Hey all,
I have a question about Real-Time Analysis of Mouse Genotypes. I need to analyze the genotypes of mice that are homozygous and heterozygous. How can I use real-time to assess genotype of the transgenic mice?

Does expression always correspond to dosage?

Southern blot analysis seems a better approach to me!... tell me please if I may be wrong.

Thanks!

Ob
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Old 05-18-2007, 06:32 AM
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Default Re: Real-Time Analysis of Mouse Genotypes

You need to know the exact location of the SNP in your sequence. Then design an assay with primers that identify SNP bases. You'll need 2 pairs. One for wild tytpe and the other one for the mutant.
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Old 05-18-2007, 06:32 AM
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Default Re: Real-Time Analysis of Mouse Genotypes

Southern blot is not very accurate and may give false positives
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Old 05-18-2007, 06:35 AM
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Default Re: Real-Time Analysis of Mouse Genotypes

If you need a software that can design SNP genotyping assays, get Beacon Designer or AlleleID. They will help you do real time PCR primer design and design of molecular probes such as TaqMan probes or molecular beacons. These primers can be used for your SNP genotyping and detection

Last edited by admin; 04-26-2008 at 08:18 AM.
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