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oBWhat 02-20-2007 03:12 AM

Real-Time Analysis of Mouse Genotypes
 
Hey all,
I have a question about Real-Time Analysis of Mouse Genotypes. I need to analyze the genotypes of mice that are homozygous and heterozygous. How can I use real-time to assess genotype of the transgenic mice?

Does expression always correspond to dosage?

Southern blot analysis seems a better approach to me!... tell me please if I may be wrong.

Thanks!

Ob

real time PCR 05-18-2007 06:32 AM

Re: Real-Time Analysis of Mouse Genotypes
 
You need to know the exact location of the SNP in your sequence. Then design an assay with primers that identify SNP bases. You'll need 2 pairs. One for wild tytpe and the other one for the mutant.

real time PCR 05-18-2007 06:32 AM

Re: Real-Time Analysis of Mouse Genotypes
 
Southern blot is not very accurate and may give false positives

real time PCR 05-18-2007 06:35 AM

Re: Real-Time Analysis of Mouse Genotypes
 
If you need a software that can design SNP genotyping assays, get Beacon Designer or AlleleID. They will help you do [Only registered and activated users can see links. Click Here To Register...] and design of molecular probes such as TaqMan probes or molecular beacons. These primers can be used for your SNP genotyping and detection


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