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problems with gDNA doing real time PCR in yeast

problems with gDNA doing real time PCR in yeast - Real-Time PCR and Quantitative PCR Forum

problems with gDNA doing real time PCR in yeast - Real-Time PCR and Quantitative PCR Forum


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Old 09-17-2013, 08:33 AM
Pipette Filler
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Default problems with gDNA doing real time PCR in yeast



Hi,

I`d like to perform real time PCR experiments in yeast. The problem is contamining genomic DNA within the isolated RNA which leads to artificial amplification products after cDNA synthesis. DNAse digestion didnt solve the problem properly and since most yeast genes dont have introns i am limited in primer design to overcome this issue.

Did anybody here make comparable experiences and has any idea how to get rid of the DNA completely or to avoid amplification from the DNA?

Thank you...
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