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-   -   qPCR Primer Product Size (http://www.molecularstation.com/forum/real-time-pcr-quantitative-pcr-forum/86884-qpcr-primer-product-size.html)

jeeep 11-27-2012 03:10 PM

qPCR Primer Product Size
 
I would like to compare the mRNA expression of 4 different receptors via Real-Time PCR. Does the product size of the qPCR primers have to be the same amongst all 4 primers? Or can they be random sizes and if so could I still quantitate the information they yield? I will be using the 2^-Delta Delta C(T) method.

For example:
Receptor 1 product = 100
Receptor 2 product = 100
Receptor 3 product = 100
Receptor 4 product = 100

or

For example:
Receptor 1 product = 106
Receptor 2 product = 150
Receptor 3 product = 111
Receptor 4 product = 100


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