Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


statistics

statistics - Real-Time PCR and Quantitative PCR Forum

statistics - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-07-2012, 10:48 AM
Volunteer
Points: 4,455, Level: 45 Points: 4,455, Level: 45 Points: 4,455, Level: 45
Activity: 99% Activity: 99% Activity: 99%
 
Join Date: Jan 2009
Posts: 31
Thanks: 1
Thanked 2 Times in 2 Posts
Default statistics



Hi everyone,

For qPCR we do 3 technical replicates and 3 biological replicates. This generates means and SDs for the technical replicates. The biological replicates are the mean of the technical replicates' means. So how do I calculate SD/SEM for the biological replicates? I've seen a lot of people ignore the SD of the technical replicates and calculate SD for the biological replicates. Surely it should be SEM as it's the mean of means?

Any help would be much appreciated.
Cat.
Reply With Quote
Reply

Tags
statistics


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Onlinetutorsite.com - Expert in math homework help, statistics homework help kst854 Article Discussion 1 04-19-2012 04:53 AM
real-time PCR statistics eej28 BioStatistics Forum 0 01-26-2010 06:54 AM
Real-time PCR statistics eej28 PCR - Polymerase Chain Reaction Forum 0 01-26-2010 06:53 AM
Statistics, Thermodynamics and Single Atoms Physics Forum 3 01-28-2006 10:34 PM
New online refresher course in statistics Statistics.Com Botany Forum 4 12-08-2003 06:28 PM


All times are GMT. The time now is 10:19 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10852 seconds with 16 queries