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#1
08-15-2012, 07:39 AM
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 how to improve limit of detection Hi there, I am trying to optimize a Taqman REaltime PCR assay with hepatitis delta viruses (RNA virus). My system generally works OK but I have to detect the lower copies of the Virus. My lower limit of detection is about 10000 copies (10^4) at the moment. Does anybody have a solution for detection of lets say 1000 (10^3) copies for instance?  |
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#2
08-15-2012, 03:48 PM
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| Re: how to improve limit of detection I am working on influenza viruses, the LOD of our assays usually are about 10-100 copies, in order to increase the LOD, just modify your primer/probe. |
| The Following User Says Thank You to namishu95 For This Useful Post: | ||
ersinka33 (04-04-2013)
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| detection , improve , limit |
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