|Register||Search||Today's Posts||Mark Forums Read|
|Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum|
| ||LinkBack||Thread Tools||Display Modes|
how to improve limit of detection
I am trying to optimize a Taqman REaltime PCR assay with hepatitis delta viruses (RNA virus). My system generally works OK but I have to detect the lower copies of the Virus. My lower limit of detection is about 10000 copies (10^4) at the moment. Does anybody have a solution for detection of lets say 1000 (10^3) copies for instance?
Re: how to improve limit of detection
I am working on influenza viruses, the LOD of our assays usually are about 10-100 copies, in order to increase the LOD, just modify your primer/probe.
|The Following User Says Thank You to namishu95 For This Useful Post:|
|detection , improve , limit|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Limit of detection||piblanlo||Real-Time PCR and Quantitative PCR Forum||6||10-07-2011 05:32 AM|
|Research News - Advanced CT with 3-D scanning improve detection of drug trafficking a||moleculardude||Science News and Views||0||05-02-2011 07:14 AM|
|PFGE - detection limit||PBT||Molecular Biology Techniques||0||05-07-2010 08:15 AM|
|PFGE - detection limit||PBT||Agarose Gel Electrophoresis Forum||0||05-07-2010 08:15 AM|
|Statistics - To little RNA or on detection limit||OliviaLarne||Real-Time PCR and Quantitative PCR Forum||3||02-17-2009 04:16 PM|