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how to improve limit of detection

how to improve limit of detection - Real-Time PCR and Quantitative PCR Forum

how to improve limit of detection - Real-Time PCR and Quantitative PCR Forum


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Old 08-15-2012, 07:39 AM
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Default how to improve limit of detection



Hi there,

I am trying to optimize a Taqman REaltime PCR assay with hepatitis delta viruses (RNA virus). My system generally works OK but I have to detect the lower copies of the Virus. My lower limit of detection is about 10000 copies (10^4) at the moment. Does anybody have a solution for detection of lets say 1000 (10^3) copies for instance?
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Old 08-15-2012, 03:48 PM
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Default Re: how to improve limit of detection

I am working on influenza viruses, the LOD of our assays usually are about 10-100 copies, in order to increase the LOD, just modify your primer/probe.
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