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-   -   how to improve limit of detection (http://www.molecularstation.com/forum/real-time-pcr-quantitative-pcr-forum/86219-how-improve-limit-detection.html)

ersinka33 08-15-2012 07:39 AM

how to improve limit of detection
 
Hi there,

I am trying to optimize a Taqman REaltime PCR assay with hepatitis delta viruses (RNA virus). My system generally works OK but I have to detect the lower copies of the Virus. My lower limit of detection is about 10000 copies (10^4) at the moment. Does anybody have a solution for detection of lets say 1000 (10^3) copies for instance?

namishu95 08-15-2012 03:48 PM

Re: how to improve limit of detection
 
I am working on influenza viruses, the LOD of our assays usually are about 10-100 copies, in order to increase the LOD, just modify your primer/probe.


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