how to improve limit of detection
 

Hi there,

I am trying to optimize a Taqman REaltime PCR assay with hepatitis delta viruses (RNA virus). My system generally works OK but I have to detect the lower copies of the Virus. My lower limit of detection is about 10000 copies (10^4) at the moment. Does anybody have a solution for detection of lets say 1000 (10^3) copies for instance?

Re: how to improve limit of detection
 

I am working on influenza viruses, the LOD of our assays usually are about 10-100 copies, in order to increase the LOD, just modify your primer/probe.