| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
05-01-2012, 06:03 PM
| |||||||||||
| |||||||||||
 influence of melting temperature of probes Hi all, I need your help. I want to desing the protocol for multiplex real-time. I have to order 3 dual-labelled probes, but one of them is much shorter (10 bp shorter) than other two (so there is a difference in melting temperature). I know that the BHQ quencher rises melting temperature, so this one I want for the shortest probe. And for two others I was thinking about eclipse quencher, but I do not know how this quencher influences the melting temperature.. Any idea? Thanks so much  |
|
| Tags |
| influence , melting , probes , temperature |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Melting temperature of the DNA and HRM | lazyphant | Real-Time PCR and Quantitative PCR Forum | 10 | 05-26-2009 06:57 AM |
| DNA melting temperature and High Resolution Melting analysis | lazyphant | DNA Forum | 0 | 04-15-2009 12:39 PM |
| Informations about melting temperature | amicia | Real-Time PCR and Quantitative PCR Forum | 4 | 02-26-2009 10:30 AM |
| GNU units and units.dat; Units of Measurement and Unit Conversion | James Redford | Physics Forum | 0 | 07-31-2005 12:08 PM |
| The Classical Vacuum - by Timothy H. Boyer | Laurent | Physics Forum | 8 | 01-14-2004 02:49 PM |
Linear Mode
