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influence of melting temperature of probes

influence of melting temperature of probes - Real-Time PCR and Quantitative PCR Forum

influence of melting temperature of probes - Real-Time PCR and Quantitative PCR Forum


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Old 05-01-2012, 06:03 PM
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Default influence of melting temperature of probes



Hi all,

I need your help. I want to desing the protocol for multiplex real-time. I have to order 3 dual-labelled probes, but one of them is much shorter (10 bp shorter) than other two (so there is a difference in melting temperature). I know that the BHQ quencher rises melting temperature, so this one I want for the shortest probe. And for two others I was thinking about eclipse quencher, but I do not know how this quencher influences the melting temperature..

Any idea?

Thanks so much
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