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QIAGEN MIDI/Minispin Kit

QIAGEN MIDI/Minispin Kit - Real-Time PCR and Quantitative PCR Forum

QIAGEN MIDI/Minispin Kit - Real-Time PCR and Quantitative PCR Forum


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Old 04-18-2012, 01:10 AM
Pipette Filler
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Default QIAGEN MIDI/Minispin Kit



Hi All,

I'm not sure if this is the best place to ask, but i'll ask anyway.

So I was extracting DNA from Serum samples and proceeded using the QIAGEN Kit, after spinning the filters with AW1 and AW2 (both wash buffers), I accidentally added SDS to the column/filter instead of Ethanol. I found out about this right after i spinned the columns through with SDS and immediately added more Ethanol (what I was supposed to add) and spinned through it again. I was planning to use the elution to run PCR and DNA analysis, but I was wondering if the addition of SDS would make this DNA useless or interfere with it too much.

ps. I added a small amount of SDS already along with the sample as part of starting material.

Any help would be greatly appreciated.

Thanks!
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Old 04-26-2012, 03:42 AM
Pipette Filler
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Default Re: QIAGEN MIDI/Minispin Kit

Hi

I read some opinions in this topic. I do not agree above ideal. We can find out some articles at about.com by using Google search.
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