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qPCR samples prepared in tube, can leave overnight before run?

qPCR samples prepared in tube, can leave overnight before run? - Real-Time PCR and Quantitative PCR Forum

qPCR samples prepared in tube, can leave overnight before run? - Real-Time PCR and Quantitative PCR Forum


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Old 10-31-2011, 05:17 PM
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Default qPCR samples prepared in tube, can leave overnight before run?



Went to use the iCycler qPCR machine and the computer that is attached to it crashed Hopefully will be fixed by tomorrow.
I have already prepared my samples for qPCR (primers, sybr green, DNA).
Can I keep my samples @ 4C overnight and run on the machine tomorrow? Or should I move them to -20C or run with fresh samples?
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Old 11-01-2011, 09:49 AM
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Default Re: qPCR samples prepared in tube, can leave overnight before run?

If you already made a PCR mix better don't store either in 4 or -20 do it freshly. i already had a bad experience by storing pcr mix. Main problem you will see big primer dimer peak in melt curve.
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