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qPCR positive controls

qPCR positive controls - Real-Time PCR and Quantitative PCR Forum

qPCR positive controls - Real-Time PCR and Quantitative PCR Forum


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Old 10-26-2011, 08:49 AM
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Default qPCR positive controls



I am running qPCR to confirm gene knock down with siRNA, and using the positive (GAPDH) and negative (scrambled) controls given by the company. I am now told by a colleague that i need a positive control containing the gene that i am interested in knocking down but my supervisor said i don't, the controls we have are enough....So who is right?
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Old 10-26-2011, 11:56 AM
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Default Re: qPCR positive controls

Your colleague is right. You need a positive control for your gene interest which you knock down. other wise how to do know your Qpcr is working in the first place.
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