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qPCR positive controls
I am running qPCR to confirm gene knock down with siRNA, and using the positive (GAPDH) and negative (scrambled) controls given by the company. I am now told by a colleague that i need a positive control containing the gene that i am interested in knocking down but my supervisor said i don't, the controls we have are enough....So who is right?
Re: qPCR positive controls
Your colleague is right. You need a positive control for your gene interest which you knock down. other wise how to do know your Qpcr is working in the first place.
|controls , positive , qpcr|
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