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why is real time pcr curve like this?

why is real time pcr curve like this? - Real-Time PCR and Quantitative PCR Forum

why is real time pcr curve like this? - Real-Time PCR and Quantitative PCR Forum


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  #1  
Old 10-21-2011, 10:51 PM
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Default why is real time pcr curve like this?



hi

I have a problem with my results of real time pcr.
I use sybr green 1 and rotor-gene (Corbett) for relative real time pcr.
Do you have any suggestion for soloution of my problem?

please look at the picture in attach

my appreciation
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File Type: jpg 1.jpg (49.1 KB, 22 views)
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Old 10-24-2011, 09:55 AM
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Default Re: why is real time pcr curve like this?

look like there is no amplification. How was the melt curve
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Old 10-24-2011, 07:06 PM
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Default Re: why is real time pcr curve like this?

please adjust the Dekta Rn( Fluorenscence)!
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