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-   -   why is real time pcr curve like this? (http://www.molecularstation.com/forum/real-time-pcr-quantitative-pcr-forum/83852-why-real-time-pcr-curve-like.html)

microbious 10-21-2011 10:51 PM

why is real time pcr curve like this?
 
1 Attachment(s)
hi

I have a problem with my results of real time pcr.
I use sybr green 1 and rotor-gene (Corbett) for relative real time pcr.
Do you have any suggestion for soloution of my problem?

please look at the picture in attach

my appreciation

obama 10-24-2011 09:55 AM

Re: why is real time pcr curve like this?
 
look like there is no amplification. How was the melt curve

namishu95 10-24-2011 07:06 PM

Re: why is real time pcr curve like this?
 
please adjust the Dekta Rn( Fluorenscence)!


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