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Probes

Probes - Real-Time PCR and Quantitative PCR Forum

Probes - Real-Time PCR and Quantitative PCR Forum


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  #1  
Old 07-14-2011, 10:13 PM
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Question Probes



I would like to know if you can use TaqMan probes and MGB probes in the same reaction, different tubes pero the same reaction? I am amplifing 2 exons of a gene using MGB and now I want to add 2 more exons but they are designed with TAMRA. Does anyone know if I can use them all together? thanks!
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Old 07-15-2011, 02:56 PM
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Default Re: Probes

yes, absolutely
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Old 07-18-2011, 05:27 PM
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Default Re: Probes

thanks....and do you know if I can use them in the same tube?
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Old 07-19-2011, 12:00 PM
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Default Re: Probes

Technically the TaqMan probes and MGB probes with different dye and close Tm can be mixed together , but you have to check primer/probe dimmer, then compare with sigplex to make sure they are not a bleed over of fluoresce
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Old 07-19-2011, 03:56 PM
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Default Re: Probes

ok thanks namishu!
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