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#1
07-14-2011, 10:13 PM
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 Probes I would like to know if you can use TaqMan probes and MGB probes in the same reaction, different tubes pero the same reaction? I am amplifing 2 exons of a gene using MGB and now I want to add 2 more exons but they are designed with TAMRA. Does anyone know if I can use them all together? thanks!   |
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#3
07-18-2011, 05:27 PM
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 Re: Probes thanks....and do you know if I can use them in the same tube? |
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#4
07-19-2011, 12:00 PM
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| Re: Probes Technically the TaqMan probes and MGB probes with different dye and close Tm can be mixed together , but you have to check primer/probe dimmer, then compare with sigplex to make sure they are not a bleed over of fluoresce |
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#5
07-19-2011, 03:56 PM
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| Re: Probes ok thanks namishu! |
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| mgb , probes , tamra |
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