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trouble shooting

trouble shooting - Real-Time PCR and Quantitative PCR Forum

trouble shooting - Real-Time PCR and Quantitative PCR Forum


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Old 07-11-2011, 06:02 PM
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Default trouble shooting



Hi there

I am designing an allele specific real time pcr to detect a mutation in the RT of HIV-1 using a dye syto9. The primers I have design are LNA primers specific for this mutation. Unfortunately i have trouble with the flourence signal. There is almost no signal. Can any one help.
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