Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


Use of end-point real-time pcr for patient follow-up

Use of end-point real-time pcr for patient follow-up - Real-Time PCR and Quantitative PCR Forum

Use of end-point real-time pcr for patient follow-up - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-19-2011, 08:15 AM
Pipette Filler
Points: 18, Level: 1 Points: 18, Level: 1 Points: 18, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2011
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Use of end-point real-time pcr for patient follow-up



Dear all,

I am a newcomer to the world of real-time PCR. Currently, I am working on the virus PCR section at a big hospital in London. We are using Taqman probes for most of our assays, which we run on 2 7500 fast instruments (applied Biosystems). For some viruses, such as respiratory viruses, we perform end-point analysis to provide a yes-no answer. The hospital has many immunocompromised in-patients that require serial monitoring in order to verify whether they have cleared the infection. My question is as follows, Is the increase in Ct values obtained on sequential samples from a patient indicative of decreasing viral load, and thus can be used to inform the clinician that the infection is clearing, or the Ct values obtained from a patient at different timepoints of their infection are not comparable when performing qualitative real-time pcr and thus only a negative-positive s interpretable.

Sorry for this long question and thank you very much

Alvi
Reply With Quote
Reply

Tags
endpoint , followup , patient , pcr , realtime


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
The Achilles Heel of String Theory. S D Rodrian Physics Forum 7 07-08-2006 02:40 PM
Moving Dimensions Theory!! Rock On!! drelliot@gmail.com Physics Forum 1 07-06-2006 05:19 PM
The Theory of Moving Dimensions: The Time Dimension is Moving Relative to The Three Spatial Dimensions Captain Ranger McCoy Physics Forum 14 07-03-2005 03:14 AM
TIME IS MOVING RELATIVE TO SPACE: Could the relative motion of dimensions unify quantumn mechanics and relativity? Dr. E Physics Forum 4 04-17-2004 09:31 PM


All times are GMT. The time now is 07:05 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13447 seconds with 16 queries