| | Re: housekeeping genes on a different plate
This topic is answering an old question I had.
So, if i understand all you said, it is possible to run all my housekeeping genes on a single plate, and then get the Ct data, and make a mean on it.
Next, I can use theses data for the normalization of all my target genes that I will run later on other PCR plates ? I do not have to run again the reference genes on each plate ?
In fact, we can run the housekeeping genes one single time, and then use the data for all the following PCR.