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what is the best housekeeping gene? - erythroid cells

what is the best housekeeping gene? - erythroid cells - Real-Time PCR and Quantitative PCR Forum

what is the best housekeeping gene? - erythroid cells - Real-Time PCR and Quantitative PCR Forum


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Old 03-17-2011, 05:58 PM
Pipette Filler
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Smile what is the best housekeeping gene? - erythroid cells



Hello everyone


I want to determine gene expression in two different sorted populations of erythroid cells (early and late erythroids), but the two housekeeping genes I have used (actin and GAPDH) are not constant among my samples. I am trying to figure out what other two housekeeping genes to try and I was planning on trying Beta-2-microglobulin and glucuronidase. Has anyone looked into expression in erythroid cells and if so, what housekeeping genes worked for you? Thanks in advance for your help.

Marilyn
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Old 01-11-2012, 07:48 AM
Pipette Filler
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Default Re: what is the best housekeeping gene? - erythroid cells

Hi
GUSB work good in blood cells, you can try it
Good luck
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cells , erythroid , gene , housekeeping


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