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-   -   what is the best housekeeping gene? - erythroid cells (http://www.molecularstation.com/forum/real-time-pcr-quantitative-pcr-forum/75887-what-best-housekeeping-gene-erythroid-cells.html)

marysb 03-17-2011 05:58 PM

what is the best housekeeping gene? - erythroid cells
 
Hello everyone


I want to determine gene expression in two different sorted populations of erythroid cells (early and late erythroids), but the two housekeeping genes I have used (actin and GAPDH) are not constant among my samples. I am trying to figure out what other two housekeeping genes to try and I was planning on trying Beta-2-microglobulin and glucuronidase. Has anyone looked into expression in erythroid cells and if so, what housekeeping genes worked for you? Thanks in advance for your help.

Marilyn

small molecule 01-11-2012 07:48 AM

Re: what is the best housekeeping gene? - erythroid cells
 
Hi
GUSB work good in blood cells, you can try it
Good luck


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