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what is the best housekeeping gene? - erythroid cells

Hello everyone

I want to determine gene expression in two different sorted populations of erythroid cells (early and late erythroids), but the two housekeeping genes I have used (actin and GAPDH) are not constant among my samples. I am trying to figure out what other two housekeeping genes to try and I was planning on trying Beta-2-microglobulin and glucuronidase. Has anyone looked into expression in erythroid cells and if so, what housekeeping genes worked for you? Thanks in advance for your help.

Marilyn

Re: what is the best housekeeping gene? - erythroid cells

Hi
GUSB work good in blood cells, you can try it
Good luck