I have some problems with my SYBR green based qPCR assay (see attachment).
When I run my qPCR assay with Sensimix SYBR I do not see any difference in the dissociation curves from the negative/NTC and positive samples. this is theoretically not possible.
The same samples/assay with Power SYBR results in two groups of dissociation curves: 1. the positive samples and 2. the negative and NTC samples. (This result is theoretically possible)
So my question is how is it possible that I see similar dissociation curves, even if there is no template involved.
Hopefully someone has some good suggestions