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Difference between SYBR mastermixes

Difference between SYBR mastermixes - Real-Time PCR and Quantitative PCR Forum

Difference between SYBR mastermixes - Real-Time PCR and Quantitative PCR Forum


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Old 03-14-2011, 01:23 PM
Pipette Filler
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Default Difference between SYBR mastermixes



Hi!

I have some problems with my SYBR green based qPCR assay (see attachment).

When I run my qPCR assay with Sensimix SYBR I do not see any difference in the dissociation curves from the negative/NTC and positive samples. this is theoretically not possible.

The same samples/assay with Power SYBR results in two groups of dissociation curves: 1. the positive samples and 2. the negative and NTC samples. (This result is theoretically possible)

So my question is how is it possible that I see similar dissociation curves, even if there is no template involved.

Hopefully someone has some good suggestions
Attached Images
File Type: jpg dissociation curves.JPG (58.1 KB, 2 views)

Last edited by fluori; 03-14-2011 at 01:27 PM.
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