Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


Unusual differences between mastermixes (Sensimix/ABI)

Unusual differences between mastermixes (Sensimix/ABI) - Real-Time PCR and Quantitative PCR Forum

Unusual differences between mastermixes (Sensimix/ABI) - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-14-2011, 09:59 AM
Pipette Filler
Points: 229, Level: 4 Points: 229, Level: 4 Points: 229, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2011
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Unusual differences between mastermixes (Sensimix/ABI)



Hi!

I'm completely new to this forum. I joined because I have some unusual dissociation curves with the sensimix SYBR. The dissociation curves from the same samples with Power SYBR are totally different.

All samples (positive, negative and NTC) give the same dissociation curves with the Sensimix SYBR, which is theoretically not possible, since the primers are specific for the positive sample and NTC/negative need to give at least different dissociation curves.
The same samples with Power SYBR mix (ABI, which is developed for our ABI Fast 7500) result in two different groups of dissociation curves: the positive samples and the negative samples/NTC, which is theoretically possible.
(see attachment)

I am wondering how it is possible that the dissociation curves of the sensimix are not specific.

Someone some suggestions??
Attached Images
File Type: jpg dissociation curves.JPG (58.1 KB, 4 views)
Reply With Quote
Reply

Tags
differences , mastermixes , sensimix or abi , unusual


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Cecità congenita e sogno Aleph Forum Biologia 53 11-06-2006 03:03 PM


All times are GMT. The time now is 04:31 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11844 seconds with 17 queries