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#1
03-10-2011, 06:39 PM
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 Data normalization Hi everyone I sorted whole bone marrow cells from a normal mouse and collected early and late erythroid cells. I then isolated RNA and determined expression of a gene my qPCR. If the cells are different (nucleated (early) versus enucleated (late)), how can I normalize this data? Should I use whole bone marrow data, or just normalize each one individually with, lets say, amount of cells? Thanks in advance for your help. Mary  |
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| data , normalization , qpcr |
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