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Data normalization

Data normalization - Real-Time PCR and Quantitative PCR Forum

Data normalization - Real-Time PCR and Quantitative PCR Forum


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Old 03-10-2011, 07:39 PM
Pipette Filler
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Question Data normalization



Hi everyone

I sorted whole bone marrow cells from a normal mouse and collected early and late erythroid cells. I then isolated RNA and determined expression of a gene my qPCR. If the cells are different (nucleated (early) versus enucleated (late)), how can I normalize this data? Should I use whole bone marrow data, or just normalize each one individually with, lets say, amount of cells?

Thanks in advance for your help.

Mary
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