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marysb 03-10-2011 07:39 PM

Data normalization
 
Hi everyone

I sorted whole bone marrow cells from a normal mouse and collected early and late erythroid cells. I then isolated RNA and determined expression of a gene my qPCR. If the cells are different (nucleated (early) versus enucleated (late)), how can I normalize this data? Should I use whole bone marrow data, or just normalize each one individually with, lets say, amount of cells?

Thanks in advance for your help.

Mary


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