Data normalization
 

Hi everyone

I sorted whole bone marrow cells from a normal mouse and collected early and late erythroid cells. I then isolated RNA and determined expression of a gene my qPCR. If the cells are different (nucleated (early) versus enucleated (late)), how can I normalize this data? Should I use whole bone marrow data, or just normalize each one individually with, lets say, amount of cells?

Thanks in advance for your help.

Mary