I sorted whole bone marrow cells from a normal mouse and collected early and late erythroid cells. I then isolated RNA and determined expression of a gene my qPCR. If the cells are different (nucleated (early) versus enucleated (late)), how can I normalize this data? Should I use whole bone marrow data, or just normalize each one individually with, lets say, amount of cells?
Thanks in advance for your help.
|All times are GMT. The time now is 06:56 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved