Originally Posted by Anais1979
I am curious - how many samples do you process for RNA extraction (Trizol) at a time?
Most people that I know don't do more than 10....
I want to do about 16 at a time, keep homogenizing and keep the homogenized ones on ice
What do you think? Good idea, bad idea?
Can it make RNA degrade?
With Trizol, do as many as you want.
I'd handle large RNA extraction with Trizol reagents like this.
Homogenize samples (usually 1ml homogenate generated).
add 200uL homogenate to 800uL Trizol, invert to mix.
Once those homogenates hit the trizol there is no degradation.
So they can sit around while the rest of the samples are processed. For the very last sample, I time 10 minutes sitting in Trizol reagent, and proceed with the extraction.
I wouldn't put the homogenate in ice, I'd do the extra step of adding/mixing it into Trizol.