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#1
08-26-2010, 07:44 AM
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 Transfection and PCR hello everyone! A question about PCR puzzled me many days! I transfected several kinds of siRNA to my tumor cell with lipofectamine. and then testify the transfection result with RT-PCR. But the problem is, the expression of negative control is always higher than that of positive control. I repeated many times,but the result was always similar. i really need your help to analyze and give me some suggestion. THANS A MILLION!!!  |
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