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Transfection and PCR
A question about PCR puzzled me many days!
I transfected several kinds of siRNA to my tumor cell with lipofectamine. and then testify the transfection result with RT-PCR. But the problem is, the expression of negative control is always higher than that of positive control.
I repeated many times,but the result was always similar. i really need your help to analyze and give me some suggestion.
THANS A MILLION!!!
|pcr , transfection|