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Transfection and PCR

Transfection and PCR - Real-Time PCR and Quantitative PCR Forum

Transfection and PCR - Real-Time PCR and Quantitative PCR Forum


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Old 08-26-2010, 07:44 AM
Pipette Filler
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Smile Transfection and PCR



hello everyone!

A question about PCR puzzled me many days!
I transfected several kinds of siRNA to my tumor cell with lipofectamine. and then testify the transfection result with RT-PCR. But the problem is, the expression of negative control is always higher than that of positive control.

I repeated many times,but the result was always similar. i really need your help to analyze and give me some suggestion.

THANS A MILLION!!!
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