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amplification curve - Real-Time PCR and Quantitative PCR Forum

amplification curve - Real-Time PCR and Quantitative PCR Forum


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  #1  
Old 08-23-2010, 12:49 PM
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Default amplification curve


Hi,
please have a look at the attached amplification curve. I don't like these. Has anyone an idea why there is a first 'bump' between cycles 4-14?
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Old 10-21-2011, 11:00 PM
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Default Re: amplification curve
maybe problem is for primer and hasnot good specificity
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Old 10-24-2011, 10:01 AM
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Default Re: amplification curve
how was the melt curve look like. any strong secondary peak
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Old 10-24-2011, 07:02 PM
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Default Re: amplification curve
how did you set up base line, from 3-15?
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