Hi guys, I met some problems in my qPCR data analysis. I wanna know expression of 10 genes. Here I performed 3 genes (including a reference gene) in a single plate for relative qPCR. Total 4 plates were used (For validating the repeatability in different plates, a gene was performed in different plate for twice). Now I need combine all the data in a single figure. But I found the expression data of the repeat gene was very different between the two plate. Is that normal? And how can I deal with it ?
ps. I used sybr green in this experiment.
Thank u, any suggestion?