Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


qPCR on different plates.

qPCR on different plates. - Real-Time PCR and Quantitative PCR Forum

qPCR on different plates. - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-20-2010, 01:00 AM
Pipette Filler
Points: 2,115, Level: 29 Points: 2,115, Level: 29 Points: 2,115, Level: 29
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question qPCR on different plates.



Hi guys, I met some problems in my qPCR data analysis. I wanna know expression of 10 genes. Here I performed 3 genes (including a reference gene) in a single plate for relative qPCR. Total 4 plates were used (For validating the repeatability in different plates, a gene was performed in different plate for twice). Now I need combine all the data in a single figure. But I found the expression data of the repeat gene was very different between the two plate. Is that normal? And how can I deal with it ?

ps. I used sybr green in this experiment.

Thank u, any suggestion?
Reply With Quote
Reply

Tags
plates , qpcr


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
qPCR NEWS January 2009 - update in available real-time PCR cycler Editor www.Gene-Quantification.info Protocols and Methods Forum 0 02-19-2009 11:04 AM
qPCR Newsletter July 2008 - main focus on qPCR optimisation Editor www.Gene-Quantification.info Protocols and Methods Forum 0 07-28-2008 02:48 PM
qPCR NEWSLETTER - September 2007 - qPCR 2007 talks online Editor www.Gene-Quantification.info Protocols and Methods Forum 0 09-25-2007 12:34 PM
qPCR NEWSLETTER - Final call for qPCR 2007 Event Editor www.Gene-Quantification.info Protocols and Methods Forum 0 02-28-2007 03:01 PM
qPCR 2007 - CALL for scientific contributions Editor www.Gene-Quantification.info Protocols and Methods Forum 0 01-22-2007 02:24 PM


All times are GMT. The time now is 01:22 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11769 seconds with 16 queries