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#1
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| Has anyone tried using EvaGreen with a Roche 480 light cycler? I tried it for the 1st time today using protocol as outlined in the instructions for EvaGreen and did not get the typical sigmoid fluorescence curve - the exponential region did not appear linear. I'd love to hear if any lightcycler users have tried this dye. Thanks, Leah Last edited by lschutt; 05-17-2010 at 05:15 AM. |
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#2
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| You can use evagreen, but you need to use the procedure compatible to Roche light cycler 480. |
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| 480 , biorad , evagreen , lightcycler , roche |
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