| | Re: Internal Standard for cytoplasmatic DNA
I think that there's a danger in using mitochondrial DNA genes as housekeeping.
A single cell can contain many mitochondria, but if you're studying one cell line you can possibly have an average number of mitochondria per cell. However, in a body different kind of cells contain different numbers of mitochondria, and so muscle cells or hepatocyte are different from e.g. epithelial cells, as far as mitochondria number is concerned.
Number of mitochondria per cell, IMO, depends on cell metabolism. If you analyse viral DNA in different tissues I think mitochondrial gene as housekeeping is not an answer.
Correct me if I'm wrong.
It doesn't matter if you fall down as long as you pick something up from the floor when you get up.