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performance of melting curve based on qPCR products

performance of melting curve based on qPCR products - Real-Time PCR and Quantitative PCR Forum

performance of melting curve based on qPCR products - Real-Time PCR and Quantitative PCR Forum


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Old 03-19-2010, 07:25 PM
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Default performance of melting curve based on qPCR products


do you know how to run a melting curve after Taqman qPCR, which plateform can be used as ,
thanks,
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Old 03-31-2010, 02:09 PM
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Default Re: performance of melting curve based on qPCR products
It's simple not possible to perform a melting curve with TaqMan probes. You can do it with intercalating dyes, FRET or molecular beacon probes, but not with TaqMan because the probes are hydrolyzed during the PCR reaction.
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Old 04-09-2010, 09:39 PM
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Default Re: performance of melting curve based on qPCR products
Melting curves with Taqman assays are not possible as the probe is destroyed during amplification.
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