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I started to work with real-time PCR quite recently so be moderate if my question seems stupid.
To get accurate results using delta delta Ct method it is necessary to have identical or very similar PCR efficiencies for target gene and endogenous control.
So does it matter if my PCR efficiency is not as good as 90 - 110 % if it is still get very similar efficiencies between my target and endogenous control, I mean values about 60 and 61 %. Or 120 and 121 % ?
|efficiency , pcr|
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