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negative control in Q-PCR how to analyze results?

negative control in Q-PCR how to analyze results? - Real-Time PCR and Quantitative PCR Forum

negative control in Q-PCR how to analyze results? - Real-Time PCR and Quantitative PCR Forum


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Old 02-27-2010, 09:39 PM
Pipette Filler
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Exclamation negative control in Q-PCR how to analyze results?



In our lab, we tried to quantify expression of a gene by q-pcr, Let's assume that the results are :

A: [1.52E6]
B: [6.54E6]
C: [7.84E6]
D: [9.82E6]
Negative: [3.10E6]

Is it appropriate to subtract the negative control from the obtained values? In case the negative control was subtracted from A, can we conclude that the gene could not be detected?(negative control is bigger than A)

thanks in advance
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