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-   -   Weird amplification curve for Taqman probes (http://www.molecularstation.com/forum/real-time-pcr-quantitative-pcr-forum/72302-weird-amplification-curve-taqman-probes.html)

fen 01-25-2010 09:35 AM

Weird amplification curve for Taqman probes
 
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Hi everyone! I m a newbie in doing qRT-PCR with Taqman probes. I encountered some weird amplification curve after i did my PCR. Firstly, the RFU signal for my amplification is quite low (app 300 RFU unit), which causing dificulties in separating the noise n the amplification curve. Secondly, I have amplification curve which kind of "floating" above the thersehold line. Is it bcoz of probe degradation? I did attached my amplification graph for a clearer pic of wat i meant.

Really need help here! :frusty:


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