Weird amplification curve for Taqman probes
Hi everyone! I m a newbie in doing qRT-PCR with Taqman probes. I encountered some weird amplification curve after i did my PCR. Firstly, the RFU signal for my amplification is quite low (app 300 RFU unit), which causing dificulties in separating the noise n the amplification curve. Secondly, I have amplification curve which kind of "floating" above the thersehold line. Is it bcoz of probe degradation? I did attached my amplification graph for a clearer pic of wat i meant.
Really need help here! :frusty:
|All times are GMT. The time now is 03:26 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved