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Real-Time PCR to detect point-mutation

Real-Time PCR to detect point-mutation - Real-Time PCR and Quantitative PCR Forum

Real-Time PCR to detect point-mutation - Real-Time PCR and Quantitative PCR Forum


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Old 11-23-2009, 08:20 PM
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Default Real-Time PCR to detect point-mutation



I have transgenic mice expressing single point mutation. I have few questions:

1. can I use real-time pcr to determine the levels of mRNA of endogenous gene and transgene in Wild Type mouse and transgenic mouse, respectively?

2. I know for WT mouse, i can use general primers (which i can design using beacon software, for eg), can I use primers (18mer) flanking around the point-mutation to do a real-time PCR for tarnsgenic samples??

In other words, can i use region-specific primers to do a real-time pcr?

Any help is appreciated!
Thanks
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Old 03-22-2010, 07:54 PM
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Default Re: Real-Time PCR to detect point-mutation

you may try MGB probe if they are morn than 3 mismatch on probe region
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