I have transgenic mice expressing single point mutation. I have few questions:
1. can I use real-time pcr to determine the levels of mRNA of endogenous gene and transgene in Wild Type mouse and transgenic mouse, respectively?
2. I know for WT mouse, i can use general primers (which i can design using beacon software, for eg), can I use primers (18mer) flanking around the point-mutation to do a real-time PCR for tarnsgenic samples??
In other words, can i use region-specific primers to do a real-time pcr?
Any help is appreciated!