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Real-Time PCR to detect point-mutation
I have transgenic mice expressing single point mutation. I have few questions:
1. can I use real-time pcr to determine the levels of mRNA of endogenous gene and transgene in Wild Type mouse and transgenic mouse, respectively?
2. I know for WT mouse, i can use general primers (which i can design using beacon software, for eg), can I use primers (18mer) flanking around the point-mutation to do a real-time PCR for tarnsgenic samples??
In other words, can i use region-specific primers to do a real-time pcr?
Any help is appreciated!
Re: Real-Time PCR to detect point-mutation
you may try MGB probe if they are morn than 3 mismatch on probe region
|detect , pcr , pointmutation , realtime|
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