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-   -   ABI 7900HT, CFX384 and Lightcycler 480 (http://www.molecularstation.com/forum/real-time-pcr-quantitative-pcr-forum/70969-abi-7900ht-cfx384-lightcycler-480-a.html)

cario 10-16-2009 12:39 PM

ABI 7900HT, CFX384 and Lightcycler 480
 
We are considering to have a 384-well qPCR system. There are three models out there, including ABI 7900HT, Biorads CFX384 and Roche Lightcycler 480. Which one you think is better?

Aga 10-16-2009 04:42 PM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
If you want a fair judgement you'll need the opinion of people that actually use those systems. An opinion on which is better will be difficult for one person, unless somebody moved from one lab to another that has different qPCR equipment.
I will be able to give you my opinion about one of the systems mentioned.

We have two qPCR systems and one of them is LC480. I was able to compare this one with capillary system and system based on peltier plate, so my opinionis rely on this only (I didn't have access to other plate systems).

Basically I like LC480 very much because it offers one of the best software that really simplifies the calculation. The visualization is also excellent.
I performed various experiments on this - very versatile, but it seems to be the feature of most qPCR systems. Basic info, such as HRM and gene scanning soft you can find using the Internet. What is really important that the system is really well equipped with soft, new features are often added, new reagents mixes, Roche's master mixes can be used to many various assays and - if needed - you can try other reagents and it works fine.

We had only one problem with lamp module - sorted within one day. And here it's quite important what kind of people work there in the service...
Bear in mind that LC480 is not the cheapest system, but I cannot compare it to other two systems you mentioned.

I hope I halped you a bit. If you meant other features let me know so I may help you more.

hkreeft 05-11-2010 05:38 PM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
hi cario,
I am having the same problem, which 1 did you decide on and why if I may ask.
I have a chromo4 now and that thing gave me nothing but trouble so I am reluctant to get another biorad product.

pretzertzumaraga 04-19-2011 08:23 AM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
I am also quite confused with what to purchase in our lab. Can you compare lightcycler and rotorgene, in terms of performance and ease of operations?Thanks a lot

namishu95 05-04-2011 12:11 PM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
with Biorads CFX384, you also may perform grdient . and i heard it will work very well for longer fragment such as 1000bp for conventional PCR, but i havenot attempted.

sunglassesatnight 05-10-2011 01:30 AM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
A good idea is to contact sales reps from each manufacturer and ask them. Of course they will only tell you what is great about their product, they will probably tell you what the problems are with the others. When you put together the thoughts from all the manufacturers, you should get a good idea of what each machine is really like.
The best thing to do though is to find labs that use each particular machine and talk to a grad student or post-doc that actually uses each one. They can give you a knowledgeable honest opinion.
Good luck with your shopping

LCMaul 06-10-2011 12:24 AM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
I am sitting in front of an AB and LC480 system right now. I can tell you what not to get:

1. Do not get the 7900 HT, that's older than dirt.
2. CFX384 is slower than dirt the second you turn on multiple channels to multiplex.
3. LC480 is my personal choice, but it suffers from information overload ... I guess that's a good problem to have but you can sit there and get lost in their naming and stuff. Not the most user friendly.
4. Let me throw in another machine in the mix : AB Viia7. I am typing next to one as we speak. Very sleek and AB really did their homework to get this one right. Its very competitive with the LC480 and urge you to give it a whirl. Its a bit expensive, but in my mind the real fight here is between :

LC480 vs ViiA 7

Dr_virologue 07-03-2011 10:00 PM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
In our lab, we have: LC, ABI7500Fast and ABI7000.

Last month, i had a training about CFX.

If i classify the three aquipement:

CFX at the first: It works silently, it doesn't need calibration aven if you transfer it in another room, and you can make a temperature gradient.

The LC: i think it's a closed system = it can work just with the Roche kits.

ABI: Need calibration after each moving, and you can import a standard curve at the end of your run for analysis.

This is my opinion

Dr_virologue 07-03-2011 10:03 PM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
Quote:

Originally Posted by namishu95 (Post 433924)
with Biorads CFX384, you also may perform grdient . and i heard it will work very well for longer fragment such as 1000bp for conventional PCR, but i havenot attempted.


:unsure: I am not agree with you! We tested the CFX with 1000pb it didn't work, but the 500pb gave better results.

namishu95 07-06-2011 12:34 PM

Re: ABI 7900HT, CFX384 and Lightcycler 480
 
great, I just heard from technical support, but have not got time to try, will perform conventional PCR with around 500 bp,


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