Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


ABI 7900HT, CFX384 and Lightcycler 480

ABI 7900HT, CFX384 and Lightcycler 480 - Real-Time PCR and Quantitative PCR Forum

ABI 7900HT, CFX384 and Lightcycler 480 - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #11  
Old 10-17-2011, 03:22 PM
Pipette Filler
Points: 52, Level: 1 Points: 52, Level: 1 Points: 52, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: ABI 7900HT, CFX384 and Lightcycler 480

Hi all,
I came across this forum while searching for a response to an issue I'm going through with my 7900HT. Hopefully, someone will have some information to share with me!
I performed a Real-Time PCR using a homemade mastermix in which I had manually added the SYBR Green I dye. Once my run had finished I could not detect any signal from my reaction.
I've read that SYBR Green I dye has spectral charateristics for which it can be detected by the argon-ion laser of the 7900HT, making them compatible. However, when reading the istruction of the 7900HT, SYBR Green I dye is always cited as a component of the SYBR Green mastermix.
So now, my point is: can I use a homemade mastermix containing a manually added SYBR Green I dye in a 7900HT instrument, or does the instrument need specifically the SYBR Green Mastermix?
Thank you!
Reply With Quote
  #12  
Old 08-20-2012, 10:41 AM
Pipette Filler
Points: 30, Level: 1 Points: 30, Level: 1 Points: 30, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2012
Posts: 15
Thanks: 0
Thanked 0 Times in 0 Posts
Default Oxygen Plants

Oxyplants India deals mainly with Oxygen plants, Nitrogen plants. We are renowned as the Manufacturer of Oxygen Plants, Nitrogen Plants and Acetylene plants.

Oxygen Plants Manufacturers
Nitrogen Plants Manufacturers
Oxygen Plants
Nitrogen Plants

For more details Visit :- oxyplants.net
Reply With Quote
  #13  
Old 08-20-2012, 10:50 AM
Pipette Filler
Points: 49, Level: 1 Points: 49, Level: 1 Points: 49, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2012
Posts: 23
Thanks: 0
Thanked 0 Times in 0 Posts
Default Turbine Ventilator Manufacturer

Good ventilation system can be a secret of success to any industry as it plays an important role in enhancing worker's efficiency & productivity. But providing good ventilation system in industrial sector becomes costly affair as it involves huge investment, energy consumption & recurring expenditure on maintenance.
Quality is a key matter on which we do not compromise. Our dedicated and result oriented team of professionals are under strict guidelines to ensure zero deficiency. Cross Vent Concept (CVC) has strong commitment to offer international quality at Indian price & service using only the world's most advanced technologies. Our skilled labour & service oriented staff are involved in maintaining high quality standards throughout the company from research and development and manufacturing departments to administrative and commercial sections.

OUR PRODUCTS :
Turbine Ventilator Manufacturer
Wind Turbine Ventilator Manufacturer
Turbo Ventilator Manufacturer
Air Ventilator Manufacturer
Radiant Barrier Manufacturer

For More Details Visit Our Site : crossventconcept.in
Reply With Quote
  #14  
Old 01-15-2013, 11:15 AM
Summer Student
Points: 222, Level: 4 Points: 222, Level: 4 Points: 222, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 86
Thanks: 0
Thanked 0 Times in 0 Posts
Default グッチ アウトレット

[

[

[
Reply With Quote
  #15  
Old 01-16-2013, 04:06 AM
Volunteer
Points: 204, Level: 4 Points: 204, Level: 4 Points: 204, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 68
Thanks: 0
Thanked 0 Times in 0 Posts
Default シャネル 店舗

[

[

[
Reply With Quote
Reply

Tags
480 , 7900ht , abi , cfx384 , lightcycler


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 01:09 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13417 seconds with 15 queries