hello guys, am a bit of a novice so just looking for some clarity.
i am undertaking rt-pcr on rat tissues, comparing four groups of animals (n=6). i am doing a taqman duplex pcr with my target gene and endogenous control.
what i was wondering was whether i need to run separate standard curves for each
of my target gene and endogenous control: ie. two curves one looking at FAM (target gene) and one looking at VIC (endogenous control) or do i need to only run a standard curve for my target gene.
thanks for the help