Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


standard curves for duplex reaction

standard curves for duplex reaction - Real-Time PCR and Quantitative PCR Forum

standard curves for duplex reaction - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-21-2009, 11:28 AM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default standard curves for duplex reaction



hello guys, am a bit of a novice so just looking for some clarity.

i am undertaking rt-pcr on rat tissues, comparing four groups of animals (n=6). i am doing a taqman duplex pcr with my target gene and endogenous control.

what i was wondering was whether i need to run separate standard curves for each of my target gene and endogenous control: ie. two curves one looking at FAM (target gene) and one looking at VIC (endogenous control) or do i need to only run a standard curve for my target gene.

thanks for the help
Reply With Quote
Reply

Tags
curves , duplex , reaction , standard


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
What does order of a reaction tell us? SNUMBER6 Chemistry Forum 29 06-25-2013 04:21 PM
No success generating standard curves... donchoi Real-Time PCR and Quantitative PCR Forum 1 11-24-2008 10:01 PM
GNU units and units.dat; Units of Measurement and Unit Conversion James Redford Physics Forum 0 07-31-2005 12:08 PM
combustion/ionisation physics Peter Lowrie Physics Forum 3 06-20-2005 01:56 PM
Standard Model Upgrading & Enhancement cinquirer Physics Forum 1 11-10-2003 03:09 AM


All times are GMT. The time now is 11:50 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13252 seconds with 16 queries