I've ran into a bit of an oddity this week. I have a SYBR Green assay where a 110bp product being made from cDNA I've used for other purposes and had no trouble with. However, I keep getting no product in my amplification plot (while other primer sets in the same assay are giving a signal). But there is seemingly a product being formed when I look at the melting curve (peaking at around 1400 RFUs).
I've also run these product using regular Taq (different thermocycler) and the product has been visible on a gel. Anyone have any ideas why this would be happening?
EDIT - this is on a Stratagene Mx3005P if that matters