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06-10-2009, 09:10 AM
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 Control for genomic contimination Hi there, i was wondering whether you think it would be acceptable to design a "gDNA control assay" (designing primers in an intron for instance), which i could use on my samples to screen for gDNA contamination, instead of amplifying a minus RT reaction (or simply RNA). It would be nice to have someone else's opinion. notworthy:  |
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06-24-2009, 11:57 AM
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| Re: Control for genomic contimination You could also look if you can find a qpcr primer (Either the forward or the reverse) that spans exonA-exonB on RNA level. (So first half of primer on exonA, second half on exonB) This way you will never amplify from genomic DNA during qpcr.  Last edited by Mathijs; 06-24-2009 at 12:00 PM. |
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| contimination , control , genomic |
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