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04-28-2009, 05:32 PM
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 cDNA bias caused by random hexamers? Hi All When using random primers to make cDNA from RNA (using Superscript III, Invitrogen) is there a bias towards either end of the RNA transcript? And how should I utilize this while designing gene specific primers during quantitave PCR with the cDNA? Is there any advantage towards using oligo dT compared to random hexamers? I think there is a bias against the 3' end because even though the primers attach anywhere along the RNA, extension will always happen towards the 5' end. Thanks a lot! Mandrita  |
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