|Register||Search||Today's Posts||Mark Forums Read|
|Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum|
| ||LinkBack||Thread Tools||Display Modes|
Rn- in during monitoring of real time pcr also in standard DAN samples
my problem is my newly designed TaqMan FAM-TAMRA probes with beacon
designer are working quite strange on standard plasmid DNA in
monolplex runs. I get negative fluorescence signals duiring
monitoring of my reaction between -30,000 to -600,000 (raw data) while working with Eppendorf real plex4. I have used the system before with TaqMan-MGB probes for the same standard DNA and had never seen such strange runs. Secondly in all pairs of primers andf probes, pcr works in one or two of replicates out of three. I can not find reason of this out put and had never before something like this also. I dono if i need to optimize the reaction or could it be a bad synthesis of probe. As far as I know FAM-TAMRA chemisdtry works well in monoplex real time pcr.
urgent assistance is acknowledged.
|-rn , dan , monitoring , pcr , real , samples , standard , std plasmid , time|
|Thread||Thread Starter||Forum||Replies||Last Post|
|the absolutely final complete collection of ideas||blochee||Physics Forum||2||06-15-2007 06:31 AM|
|Moving Dimensions Theory!! Rock Onfirstname.lastname@example.org||Physics Forum||1||07-06-2006 05:19 PM|
|The Theory of Moving Dimensions: The Time Dimension is Moving Relative to The Three Spatial Dimensions||Captain Ranger McCoy||Physics Forum||14||07-03-2005 03:14 AM|