I hope someone can help me whit this.
Im doing a research in humans so im using a sample of lymphocytes and im doingo real time PCR. Using a GRS primers and 36B4Ribosomal as a housekeeping.
I got contamination, my negative amplifies and my syber green, the water and the primers got contamination every time. I change everything all the time but im tired to got this so often. So i decided to work on the laminar flow cabinet. And a got contamination so i dont whats happening and dont know what else to do.
Can any body help me? some ideas??? i try almost everything