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no band is detectable in gel

no band is detectable in gel - Real-Time PCR and Quantitative PCR Forum

no band is detectable in gel - Real-Time PCR and Quantitative PCR Forum


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Old 02-01-2009, 12:38 PM
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Default no band is detectable in gel



How much fluorescence intensity in real time PCR reaction usually gives a detectable band in agarose gel electrophoresis? I usually got very typical sigmoidal curve of real time PCR with clear Ct value and acceptable MT curve, but no band is detectable in gel, do you have any idea?
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