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no band is detectable in gel

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no band is detectable in gel

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02-01-2009, 11:38 AM

siamak

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no band is detectable in gel

How much fluorescence intensity in real time PCR reaction usually gives a detectable band in agarose gel electrophoresis? I usually got very typical sigmoidal curve of real time PCR with clear Ct value and acceptable MT curve, but no band is detectable in gel, do you have any idea?

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band , detectable , detectable band in gel , fluorescence intensity , gel

« White or Clear PCR plates? | For all primers I get a additional peak in MT curve at 77 »

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no band is detectable in gel

no band is detectable in gel - Real-Time PCR and Quantitative PCR Forum

no band is detectable in gel - Real-Time PCR and Quantitative PCR Forum