Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum > Real-Time PCR and Quantitative PCR Forum
Register Search Today's Posts Mark Forums Read

Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum


White or Clear PCR plates?

White or Clear PCR plates? - Real-Time PCR and Quantitative PCR Forum

White or Clear PCR plates? - Real-Time PCR and Quantitative PCR Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-27-2009, 12:58 PM
esl esl is offline
Pipette Filler
Points: 3,150, Level: 36 Points: 3,150, Level: 36 Points: 3,150, Level: 36
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2008
Posts: 15
Thanks: 0
Thanked 1 Time in 1 Post
Default White or Clear PCR plates?



Hello,

I'm just wondering if you can advise me on choosing plates for real-time PCR. I've heard that white plates can give better sensitivity and improve the signal:noise ratio.

We have some previous data which came from white plates and showed a 20-fold difference between treatments, but new data from clear plates which shows no difference. Could the plate difference be the answer - we can't find any other explanation and the RNA came from the same tissue.

Thanks for your help!
Reply With Quote
  #2  
Old 01-27-2009, 09:42 PM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: White or Clear PCR plates?

According to the manufacturer of our real-time system (Roche - we have LightCycler 480 for plates analysis) white plates are better unless you have software version 1.5 and above. With newer software version you can use both types of plates and no changes should be observed.

So I don't know if it's the software issue but it could be so - it depends on the software how the fluorescence sample to background ratio is interpreted and so on. I guess it could also depend on the type of real-time system.

I use white plates if anyone asks
Reply With Quote
Reply

Tags
clear , pcr , plates , white


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
PAGE gel sticked on both plates Patricia C Arbour-Reily Protocols and Methods Forum 0 12-08-2008 03:41 PM
Bin Laden family, Saudi Arabia corruption and support of terrorists, connections to Bush... Laurent Physics Forum 3 06-22-2004 09:31 AM
The Classical Vacuum - by Timothy H. Boyer Laurent Physics Forum 8 01-14-2004 03:49 PM
What is this white fluffy stuff made by adding vinegar to goldchloride/goldiodide? Vlad Chemistry Forum 7 12-23-2003 12:31 AM


All times are GMT. The time now is 03:10 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13174 seconds with 16 queries