I work with a native tobacco species, and I´m having some trouble with my RT-PCR. I use SYBR green, and I´m seeing high fluorescence at the firsts cycles, then, a rise near the Ct and the normal declination during the melting curve stage. The amplification plot seems to be ok and the dissociation plot too. The only problem seems to be the fluorescence plot, it´s like if I have a lot of dsDNA at the begginig, but I´ve run my RT in a gel and there is not any band of genomic DNA. Does anyone know what could be? I´dont know if I can trust in the Ct values, having a basal fluorescence too high like this.