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Real-time PCR Primer Design Software

Real-time PCR Primer Design Software - Real-Time PCR and Quantitative PCR Forum

Real-time PCR Primer Design Software - Real-Time PCR and Quantitative PCR Forum


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  #1  
Old 10-10-2006, 07:10 PM
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Default Real-time PCR Primer Design Software



What software is out there for real-time pcr primer design?

any free ones? and what do you guys use?

thanks
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  #2  
Old 11-18-2006, 09:59 PM
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Default Re: Real-time PCR Primer Design Software

Beacon Designer 6.0
cracked one
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  #3  
Old 11-20-2006, 08:28 AM
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Default Re: Real-time PCR Primer Design Software

DNAStar Lasergene 7
Vector NTI

Vector NTI u can download for free from
_ttps://catalog.invitrogen.com/index.cfm?fuseaction=userGroup.downloadCenter

Receive license by active Invitrogen account. its order just as product (PC cat# 12605112, MAC cat# 12605113) Instead of "P.O.#" u need to write "open access"

Last edited by Leowe; 11-20-2006 at 09:25 AM.
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Old 12-07-2007, 06:22 PM
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Default Lasergene Software

Someone has the SN of Lasergene 7.2.1.?
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  #5  
Old 12-07-2007, 06:50 PM
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Default Re: Real-time PCR Primer Design Software

Quote:
Originally Posted by oBWhat View Post
What software is out there for real-time pcr primer design?
OLIGO from Molecular Biology Insights, the best in my opinion, check out the features at ---> [Only registered users see links. ]
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Old 01-15-2008, 09:42 AM
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Default Re: Real-time PCR Primer Design Software

beacon designer 7.0
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  #7  
Old 02-05-2008, 02:53 PM
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Default Re: Real-time PCR Primer Design Software

beacon designer 7.0, simple the best
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  #8  
Old 02-28-2008, 08:09 AM
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Default Re: Real-time PCR Primer Design Software

Beacon Designer 7 is sure the most comprehensive one. Other tools such as Oligo either do not confirm to the qpcr standards or do not have major supporting chemistries.

One thing always to look out for is how well the authoring supports. Going by my experience this vendor (Premier Biosoft) does a pretty decent job. They also offer qpcr assay design services for you if your design is too difficult and still you won't like to shed that much money. This is good if I am designing multiplex PCR assays or alignment assays (cross species or specific to a single sequence).

VectorNTI is ok for planning cloning but for primer and probe design, I personally would never compromise and choose the best.

Last edited by admin; 04-26-2008 at 08:16 AM.
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  #9  
Old 04-25-2008, 02:29 PM
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Default Re: Real-time PCR Primer Design Software

i think Primer Premier 5 is the best ,but lasergene is fine.
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Old 12-10-2008, 02:25 PM
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Default Re: Real-time PCR Primer Design Software

Hello
We are using the Roche universal probes design tool - our primers are always really good efficiency, even with SYBR green
Good luck!
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