Real-time PCR Primer Design Software

[oBWhat]

What software is out there for real-time pcr primer design?

any free ones? and what do you guys use?

thanks

[Leowe]

Beacon Designer 6.0
cracked one

[Leowe]

DNAStar Lasergene 7
Vector NTI

Vector NTI u can download for free from
_ttps://catalog.invitrogen.com/index.cfm?fuseaction=userGroup.downloadCenter

Receive license by active Invitrogen account. its order just as product (PC cat# 12605112, MAC cat# 12605113) Instead of "P.O.#" u need to write "open access"

[Susanna]

Someone has the SN of Lasergene 7.2.1.?

[danfive]

Originally Posted by oBWhat

What software is out there for real-time pcr primer design?

OLIGO from Molecular Biology Insights, the best in my opinion, check out the features at ---> [Only registered users see links. ]

[angelbaobao]

beacon designer 7.0

[amicia]

beacon designer 7.0, simple the best

[real time PCR]

Beacon Designer 7 is sure the most comprehensive one. Other tools such as Oligo either do not confirm to the qpcr standards or do not have major supporting chemistries.

One thing always to look out for is how well the authoring supports. Going by my experience this vendor (Premier Biosoft) does a pretty decent job. They also offer qpcr assay design services for you if your design is too difficult and still you won't like to shed that much money. This is good if I am designing multiplex PCR assays or alignment assays (cross species or specific to a single sequence).

VectorNTI is ok for planning cloning but for primer and probe design, I personally would never compromise and choose the best.

[tiangua997]

i think Primer Premier 5 is the best ,but lasergene is fine.

[esl]

Hello
We are using the Roche universal probes design tool - our primers are always really good efficiency, even with SYBR green
Good luck!