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Melt Curve Result

Melt Curve Result - Real-Time PCR and Quantitative PCR Forum

Melt Curve Result - Real-Time PCR and Quantitative PCR Forum


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Old 12-03-2008, 03:10 AM
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Question Melt Curve Result



I have been doing some cocktail mastermix for realtime pcr. however most the time i am getting the bell shape peak in the negative region as shown in the attachment instead of the positive region. can anybody explain why does this occur? do i need to change something to make it to the positive region or just retain it. the peak is at a very consistence temperature say 87C
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Old 12-08-2008, 03:49 PM
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Default Re: Melt Curve Result




I will look into this for you Butters...
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Old 12-08-2008, 03:52 PM
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Default Re: Melt Curve Result

thank a lot admine! i really appreciate it
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