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Problems with second use of diluted cDNA in qPCR

Problems with second use of diluted cDNA in qPCR - Real-Time PCR and Quantitative PCR Forum

Problems with second use of diluted cDNA in qPCR - Real-Time PCR and Quantitative PCR Forum


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Old 09-30-2008, 06:07 PM
Pipette Filler
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Default Problems with second use of diluted cDNA in qPCR



Hello All,

Each time that i am using diluted cDNA in my reaction, i am getting a good results.

However, when i use the SAME (from the same tube) diluted cDNA, the results looks like a noise!

I have tried to store the samples in -20C or +4C, the results remain the same. Moreover, during the preparations of the qpcr samples the cDNA has been stored on ice.

The Problem occur consistently.

May be, someone has an idea why does it happening??

Please Help!

Regards,

Anat
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