| |||||||
| Register | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum |
 real-time Videos | |
| |
 |
 |
| | LinkBack | Thread Tools | Display Modes |
09-30-2008, 07:07 PM
| |||||
| |||||
 Problems with second use of diluted cDNA in qPCR Hello All, Each time that i am using diluted cDNA in my reaction, i am getting a good results. However, when i use the SAME (from the same tube) diluted cDNA, the results looks like a noise! I have tried to store the samples in -20C or +4C, the results remain the same. Moreover, during the preparations of the qpcr samples the cDNA has been stored on ice. The Problem occur consistently. May be, someone has an idea why does it happening?? Please Help! Regards, Anat  |
| Today
| ||||
| ||||
 Sponsored Links |
|
| Tags |
| cdna , diluted , problems , qpcr |
| Thread Tools | |
| Display Modes | |
|
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| cDNA Synthesis and Amplification of POLY(A) mRNA by RT-PCR Protocol | domba | Real-Time PCR and Quantitative PCR Forum | 3 | 09-09-2008 03:48 PM |
| Problems with RNA extraction or QPCR for AI | deki | Real-Time PCR and Quantitative PCR Forum | 2 | 12-30-2007 08:58 PM |
| Commercial cDNA or cdna Synthesized Random hemer for Transfection | bhunni | DNA Techniques | 0 | 09-01-2007 08:12 AM |
| Problems with RNA extraction or QPCR for AI | deki | Basic Lab Protocols and Techniques | 3 | 07-27-2007 03:59 AM |
| cdna | bhunni | DNA Techniques | 0 | 06-26-2007 09:45 AM |
Linear Mode
