| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Real-Time PCR and Quantitative PCR Forum Real-Time PCR and Quantitative PCR Forum |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
09-30-2008, 06:07 PM
| |||||||||||
| |||||||||||
 Problems with second use of diluted cDNA in qPCR Hello All, Each time that i am using diluted cDNA in my reaction, i am getting a good results. However, when i use the SAME (from the same tube) diluted cDNA, the results looks like a noise! I have tried to store the samples in -20C or +4C, the results remain the same. Moreover, during the preparations of the qpcr samples the cDNA has been stored on ice. The Problem occur consistently. May be, someone has an idea why does it happening?? Please Help! Regards, Anat  |
|
| Tags |
| cdna , diluted , problems , qpcr |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| qPCR NEWS January 2009 - update in available real-time PCR cycler | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 02-19-2009 10:04 AM |
| qPCR Newsletter July 2008 - main focus on qPCR optimisation | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 07-28-2008 02:48 PM |
| qPCR NEWSLETTER - September 2007 - qPCR 2007 talks online | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 09-25-2007 12:34 PM |
| qPCR 2007 - CALL for scientific contributions | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 01-22-2007 01:24 PM |
| qPCR NEWSLETTER - October 2006 | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 10-26-2006 12:25 PM |
Linear Mode
