Hi Everyone. First time for me to write in such forum. I am now doing qPCR (learning actually) and I have a question to anyone who might be able to help.
I am using the standart curve method to determine my primers efficiency. It works like this:
- I am making series of standart dilutions (i.e. 1:5)
- than the efficiency is calculated in % from the slope of the curve that I get (i.e. 95%).
The thing is that I am not sure above which value of PCR efficiency is my experiment considered 'good'. Is it ok if the efficiency is about 90 % ? Or does it have to be 100 % ? Can I use the primers under the same conditions if the efficiency calculated by this method happens to be below 90 % ?