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Contamination of Real-time PCR

Contamination of Real-time PCR - Real-Time PCR and Quantitative PCR Forum

Contamination of Real-time PCR - Real-Time PCR and Quantitative PCR Forum


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  #1  
Old 09-08-2006, 07:45 PM
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Arrow Contamination of Real-time PCR



Hey guys,

I think i may have contamination in my real time pcr... how do you know this for sure?

are there any signs or ways to tell? ie peaks are off?

thanks
Kiki
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Old 07-20-2007, 02:39 PM
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Default Re: Contamination of Real-time PCR

It depends on the shape of the amplificaiton curve of your house keeping gene. What is the CT value of the house keeping gene of your sample? or could you show the picture of the amplication curve?
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Old 07-23-2007, 04:00 PM
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Default Re: Contamination of Real-time PCR

Run negative controls. If using SYBR green determine melting points. If fluroescent probes you can run your reaction on a 2-3% agarose gel.
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